To determine whether myocyte death and angiotensin ii at ii formation are implicated in the development of diabetic cardiomyopathy, rats were injected with streptozotocin, and apoptosis and. Induction of diabetes by streptozotocin in r ats article pdf available in indian journal of clinical biochemistry 22222. The effect of phaseolus vulgaris, an indigenous plant used in ayurvedic medicine in india, on circulatory antioxidants and lipids was studied in rats with streptozotocininduced diabetes. Evaluation of sphingolipid metabolism in renal cortex of. Old wistar rats with streptozotocin induced diabetes were randomly divided into three groups. Diabetes was induced in male wistar rats by the administration of stz 60. Compared to normal nondiabetic rats, stz induced diabetic rats had approximately 200% higher serum glucose, 50% higher serum cholesterol and 30% higher serum triglyceride levels as well as 86% higher urinary protein levels. Streptozotocin or streptozocin inn, usp stz is a naturally occurring alkylating antineoplastic agent that is particularly toxic to the insulinproducing beta cells of the pancreas in mammals. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t. Intravenous injection of 60mgkg dose of streptozotocin in adult wistar rats, makes pancreas swell. In conclusion, the streptozotocininduced diabetes mellitus in rats was stable for 6 months, afterward, however, a significant recovery developed in. Stzinduced diabetes streptozotocin stz is an alkylating agent that targets insulinproducing beta cells in the pancreas, mimicking type 1 diabetes phenotypes in mice. Induction of diabetes by streptozotocin in rats ncbi nih. Nutrients free fulltext caffeine at a moderate dose did.
Kurtansky2 1department of pharmacology and 2department of physiology, faculty of medicine, comenius university, 3institute of experimental pharmacology, slovak academy of sciences, bratislava. Naringenin was found to protect rat kidney in streptozotocin stzinduced diabetes by altering oxidative stress, modulation of cytokines expression tgf. Stz is well known to cause pancreatic bcell damage, whereas. To better understand the insulinindependent plasma glucoselowering action of metformin, we used streptozotocin stzinduced diabetic rats to investigate the possible mechanisms. In this study, diabetes was induced by a single injection of streptozotocin stz, 45 mgkg, i. To determine whether myocyte death and angiotensin ii at ii formation are implicated in the development of diabetic cardiomyopathy, rats were injected with streptozotocin, and apoptosis. Effect of streptozotocin on glucose levels in albino wister rats. To understand the pathophysiological mechanisms and factors involved in diabetes, the use of pancreatic regeneration studies is increasing in an attempt to understand the behavior of pancreatic beta cells.
Although streptozotocin administered after nicotinamide induced slight increases in glucose levels at the beginning of the experiment only, slight, but significant unfavorable changes in the skeletal system were demonstrated. Streptozotocininduced diabetes in the rat is associated with. Diabetes was induced in male wistar rats by the administration of stz 60 mgkg b. We examined the effects of photobiomodulation pbm on stereological parameters, and gene expression of runtrelated transcription factor 2 runx2, osteocalcin, and.
The effect of streptozotocininduced diabetes in rats on. Guangyi liu, fei han, yi yang, yi xie, hong jiang, youying mao, huiping wang, minmin wang, rongjun chen, jun yang, jianghua chen, evaluation of sphingolipid metabolism in renal cortex of rats with streptozotocininduced diabetes and the effects of rapamycin, nephrology dialysis transplantation, volume 26, issue 5, may 2011, pages 14931502. Administration of both streptozotocin stz and nicotinamide na has been proposed to induce experimental diabetes in the rat. Pdf streptozotocinnicotinamideinduced rat model of. Effects of stzinduced diabetes on the relative weights of kidney, liver and pancreas in albino rats. Streptozotocin use in mice and rats version 1 date of publication. On the other hand, the use of high stz doses eg, 100 mgkg for rats could. Murugesan2 1department of biochemistry, kanchi shri krishna college of arts and science, kilambi. Evaluation of sphingolipid metabolism in renal cortex of rats. In the present study streptozotocin dose was as 50mgkg body weight in study of zafar16, m et al used 50mgkg bw stz dose, in study of mozaffari et al23 used 90 mgkg body weight. Background diabetes mellitus is an endocrine metabolic disorder of. To understand the pathophysiological mechanisms and factors. Administration of caffeine did not affect the investigated skeletal parameters of rats with streptozotocin induced. Streptozotocin stz is a widely used chemical for the induction of experimental diabetes in rodents.
Full text streptozotocininduced type 1 diabetes in rodents as a. Old wistar rats with streptozotocininduced diabetes were randomly divided into three groups. Nutrients free fulltext caffeine at a moderate dose. Sulodexide improves endothelial dysfunction in streptozotocin. For induction of experimental diabetes in male adult rats weighted 250300 grams 7590 days, 60mgkg of streptozotocin was injected intravenously. Results effects of metformin on plasma glucose and ber levels in stzinduced diabetic rats. This study investigated the role of heme oxygenase ho1 in the cardiac tissue injury of acute ischemiareperfusion ir in diabetic streptozotocin stz induced hyperglycemic rats. This difference can be significant, with little or no response in female mice and severe hyperglycemia present in male mice receiving identical doses.
Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Rats treated with stz and na manifest symptoms of type 2 diabetes, while animals with stzinduced type. Streptozotocininduced diabetes in the rat is associated. There are no documented studies, available in the literature that showed the histopathological changes in the liver tissue of.
The functional insufficiency of the recovery in this period 712 months after streptozotocin application may be uncovered by the glucose tolerance test. Research open access metabolomic analysis of rat serum in. Effect of bauhinia holophylla treatment in streptozotocininduced. Streptozotocininduced diabetic neuropathy in mice and rats. Antidiabetic and hypolipidaemic properties of garlic. Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received. Research open access metabolomic analysis of rat serum.
Effects of stzinduced diabetes on the relative weights of. In control and streptozotocin treated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase i, endothelial nitric oxide synthase enos and cgmp. Protective effect of cynodon dactylon aqueous extract in. Compared to normal nondiabetic rats, stzinduced diabetic rats had approximately 200% higher serum glucose, 50% higher serum cholesterol and 30% higher. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. Antidiabetic and hypolipidaemic properties of garlic allium.
It has been successfully induced in a variety of animal species. Streptozotocin is a glucosaminenitrosourea compound. As with other alkylating agents in the nitrosourea class, it is toxic to cells by causing damage to the dna, though other mechanisms may also contribute. Stz diabetic rats were treated with oral doses of mebs 200 and 400 mgkg for 8 weeks. Effect of streptozotocin on glucose levels in albino. Pdf induction of diabetes by streptozotocin in rats researchgate. The aim of this study was to evaluate the antihyperglycemic effects of gbe on streptozotocin stz induced diabetes in rats.
The effects of 1 hemin, an inducer of ho expression and activity, and 2 zinc protoporphyrin ix znppix, an inhibitor of ho activity, have also been investigated on the tissue injury by ir and some. The prevalence, and associated healthcare burden, of diabetes mellitus is increasing worldwide. Antidiabetic effects of aqueous extract and oil of moringa. Critical evaluation of the streptozotocin model of painful diabetic neuropathy in the rat. Sulodexide improves endothelial dysfunction in streptozotocininduced diabetes in rats v. Effect of aqueous extract and oil of moringa oleifera seed on blood glucose, serum alt and ast activities in streptozotocin induced diabetic rats prolonged treatment with aqueous extract and oil of moringa oleifera seed has shown a significant decrease p0.
Background diabetes mellitus is an endocrine metabolic disorder of impaired carbohydrate, fat and protein metabolisms characterized by chronic hyperglycemia. Diabetes mellitus is a clinical disorder in which blood sugar levels are abnormally high known as hyperglycemia. The effect of phaseolus vulgaris, an indigenous plant used in ayurvedic medicine in india, on circulatory antioxidants and lipids was studied in rats with streptozotocin induced diabetes. Coffee drinking, apart from its health benefits, is taken into consideration as an osteoporosis risk factor. Stz is well known to cause pancreatic bcell damage, whereas na is ad. Oral intake of metformin decreased the plasma glucose of stzinduced diabetic rats with a parallel increase of plasma.
The mechanism of alloxan and streptozotocin action in b cells. The full text of this article is available as a pdf 180k. Skeletal system of rats with streptozotocininduced diabetes. Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with nonketosis hyperglycemia in some animal species. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water. Guangyi liu, fei han, yi yang, yi xie, hong jiang, youying mao, huiping wang, minmin wang, rongjun chen, jun yang, jianghua chen, evaluation of sphingolipid metabolism in renal cortex. There are no documented studies, available in the literature that showed the histopathological changes in the liver tissue of streptozotocin induced diabetic rats treated with aqueous extract of cynodon dactylon, which made us to conduct this study. Antihyperglycemic effect of ginkgo biloba extract in. However, the effects of diabetes on genital tissue structure, innervation and function remains poorly characterized. Streptozotocininduced diabetic models in mice and rats. Streptozotocinnicotinamideinduced diabetes in the rat. We examined the effects of photobiomodulation pbm on stereological parameters, and gene expression of runtrelated transcription factor 2 runx2, osteocalcin, and receptor activator of nuclear factor kappab ligand rankl in repairing tissue of tibial bone defect in streptozotocin stz induced type 1 diabetes mellitus tidm in rats during catabolic response of fracture healing.
The effect of streptozotocininduced diabetes in rats. The mechanism of alloxan and streptozotocin action in b. Evaluation of the effects of photobiomodulation on partial. Alloxan has been administered in single or multiple doses, through different routes intraperitoneal. Antidiabetic effects of aqueous extract and oil of. Propel your preclinical research using jax inventoried, ondemand, and unique stzinjected models generated on various genetic backgrounds, as well as additional services. From the above results it is shown that the treatment. Effect of phaseolus vulgaris on circulatory antioxidants.
Dna damage induces activation of parp which is likely more important for diabetes induction than the dna damage itself. Alloxaninduced diabetes, a common model for evaluating. Streptozotocin stz is an antibiotic that produces pancreatic islet cell destruction and is widely used experimentally to produce a model of type 1 diabetes mellitus t1dm. Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with nonketosis hyperglycemia in some.
Type 1 diabetes t1d is a pathological condition characterized by the loss of insulinproducing. In this view novel synthetic benzopyrone analogues were selected for testing in experimental diabetes. Ameliorative potential of morin in streptozotocininduced neuropathic pain in rats. Streptozotocininduced type 1 diabetes in rodents as a. Pdf antidiabetic activity of benzopyrone analogues in. Stz induced diabetes are commonly performed in male wistar kyoto rats. Ameliorative potential of morin in streptozotocin induced neuropathic pain in rats. Oxidative stress parameters, namely, serum superoxide dismutase, catalase. Oxidative stress parameters, namely, serum superoxide dismutase, catalase, malondialdehyde and fructosamine in diabetic rats were reverted to respective normal values after glycyrrhizin administration. Antidiabetic effect of betulinic acid on streptozotocin. In these models, male wistar rats at 8 weeks of age 200300 g are selected from animal supply facility of sgpgims lucknow, and. Induction of diabetes by streptozotocin in rats springerlink.
Stz induced diabetes streptozotocin stz is an alkylating agent that targets insulinproducing beta cells in the pancreas, mimicking type 1 diabetes phenotypes in mice. Modeling type 2 diabetes in rats using high fat diet and. Diabetes may lead to the development of osteoporosis. Benzopyrones are proven antidiabetic drug candidate in diabetic drug discovery. Ameliorative effects of glycyrrhizin on streptozotocin. Pdf induction of diabetes by streptozotocin in rats. After treatment, in the pancreas no insulitis was observed. Stz initiates destruction of langerhans islets cells irreversibly and induces diabetes in rats 15. Effect of phaseolus vulgaris on circulatory antioxidants and.
For induction of experimental diabetes in male adult rats. Calcium atpase activity of cardiac actomyosin was significantly decreased after 1 week of diabetes and was depressed by 60% by 2 weeks. Male mice and rats tend to be more susceptible to stzinduced diabetes 10, 15, 16, 2225. Rodents also show a substantial gender difference in stz sensitivity. Diabetes is associated with declining sexual function in women. Diabetes was induced by streptozotocin 40 mgkg in female wistar rats. The neurological consequences of diabetes mellitus in the brain have gained attention most recently. Effect of aqueous extract and oil of moringa oleifera seed on blood glucose, serum alt and ast activities in streptozotocininduced diabetic rats prolonged treatment with aqueous extract and oil of. Type 2 diabetes t2d was induced in wistar rats by streptozotocin. At 60 min after treatment, a dosedependent decrease of plasma glucose was observed in stz. Myocyte death in streptozotocininduced diabetes in rats. In the present study streptozotocin dose was as 50mgkg. Myocyte death in streptozotocininduced diabetes in rats is.
Pdf effect of streptozotocin induced diabetes on rat. Objective the present study was designed to evaluate the diabetes induced neuronal damage in the hippocampus of the rat brain. Streptozotocininduced diabetes mellitus an overview. In these models, male wistar rats at 8 weeks of age 200300 g are selected from animal supply facility of sgpgims lucknow, and house in a 12h light, 12h dark cycle environment, temperature 25c, with standard rat diet and water in metabolic cages for one week prior to. Bauhinia holophylla leaves treatment on the streptozotocininduced diabetic rats. Jan 14, 2011 the treatment significantly reduced diabetes. Streptozotocin stzinduced diabetic animal models are useful. Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals.
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